Translational_Unit

Part:BBa_K3050000:Design

Designed by: Kita Mizuki   Group: iGEM19_Gunma   (2019-10-15)


pT7+RBS+mag-2+GFP+double_terminator


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 190


Design Notes

We planned to gain linearized vector by conducting inverse PCR to BBa_I746909 between RBS and GFP so that our original part,mag-2,can be put into between RBS and GFP.


Source

Magainin-2 is toxic protein for microorganisms that derives from Xenopus laevis's skin. Magainin-2 damages cell membrane.

References